The Amazing Spider-Man #129 CGC 5.5
1st app of the Punisher (Frank Castle).
1st app of Miles Warren as the Jackal, see note below.
"The Punisher Strikes Twice!" – (19-page story) – A new costumed being known as the Jackal has appeared and has hired the Punisher -- a costumed vigilante that loves death -- to destroy Spider-Man. The wall-crawler, is meanwhile web-slinging through the city, he stops to take some pictures of a robbery and busts it up as well. He takes the photos to the Bugle, where Jameson has a fit that Parker has not been able to get any photos of the Punisher, and that all the competition is snapping up photos of him in action. ...
NOTE: Miles Warren first app was as himself in ASM #31 (Dec. 1965).